2015 – Volume 4, Issue 1 / (Page 13-17)

Effects of sublethal concentrations of propargite to white blood cells of common carp (Cyprinus carpio, Linnaeus, 1758)

Arinc Tulgar, Ekrem Sanver Celik

 

Abstract


In this study, effects of propargite, an organosulphiric pesticide were investigated in carp’s (Cyprinus carpio) white blood cells such as lymphocyte (LYM), neutrophile (NEU), monocyte (MON), basophile (BAS) and eosinophile (EOS). Fish were exposed to control (only distilled tap water), low (0.04125 ppm), medium (0.0825 ppm) and high (0.165 ppm) concentrations of propargite. As a result of study, percentage of BAS, NEU and MON cells were generally showed an increase on the 14th day compared to the control group, in case percentage of LYM and EOS cells were decreased on the 14th day according to the control group. In conclusion, changes observed among the white blood cell types could demonstrate the immunosupresive effect of propargite.

 

Keywords

Propargite, Carp, White blood cell types

 

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References

  • Adhikari, S., Sarkar, B., Chatterjee, A., Mahapatra, C.T. and S. Ayyappan. 2004. Effects of cypermethrin and carbofuran on certain hematological parameters and prediction of their recovery in a freshwater teleost, Labeo rohita (Hamilton). Ecotoxicology and Environmental Safety, 58:220-226.
  • Ahmad, Z. 2011. Acute toxicity and hematological changes in common carp (Cyprinus carpio) caused by diazinon exposure. African Journal of Biotechnology, 10(63):13852-13859.
  • APHA, AWWA and WEF. 1998. Standard methods for the examination of water and wastewater, Retrieved January 5, 2012, from https://law.resource.org/pub/us/cfr/ibr/002/apha.method.3111.1992.pdf.
  • Chandrasekar, S. and N. Jayabalan. 1993. Hematological responses of the common carp, Cyprinus carpio L. exposed to the pesticide endosulfan. Asian Fisheries Science, 6:331-340.
  • Esteban, M.A., Munoz, J. and J. Meseguer. 2000. Blood cells of sea bass (Dicentrarchus labrax L.). Flow cytometric and microscopic studies. The Anatomical Record, 258:80-89.
  • Fırat, Ö., Cogun, H.Y., Yüzereroğlu, T.A., Gök, G., Fırat, Ö., Kargin, F. and Y. Kötemen. 2011. A comparative study on the effects of a pesticide (Cypermethrin) and two metals (Copper, Lead) to serum biochemistry of Nile tilapia, Oreochromis niloticus. Fish Physiology Biochemistry, 37:657-666.
  • Gholami-Seyedkolaei, S.J., Mirvaghefi, A., Farahmand, H. and A.A. Kosari. 2013. Effect of a glyphosate-based herbicide in Cyprinus carpio: Assessment of acetylcholinesterase activity, hematological responses and serum biochemical parameters. Ecotoxicology and Environmental Safety, 98:135-141.
  • Giron-Perez, M.I., Barcelos-Garcia, R., Vidal-Chavez, Z.G., RomeroBanuelos, C.A. and M.L. Robledo-Marenco. 2006. Effect of chlorpyrifos on the hematology and phagocytic activity of Nile tilapia cells (Oreochromis niloticus). Toxicology Mechanisms and Methods, 16:495-499.
  • Harford A.J., O’Halloran K., Wright P.F., 2005. The Effects of in vitro Pesticide Exposures on the Phagocytic Function of Four Native Australian Freshwater Fish. Aquatic Toxicology, 75: 330-342.
  • Ivan, G.P.M., Anne, S., Fabiola, G.J., Josefina, C.S., Marcela, H.C., Jorge, P.S., Akiro, T. and Z. Galina. 2007. Immunotoxicity and hepatic function evaluation in Nile tilapia (Oreochromis niloticus) exposed to diazinon. Fish and Shellfish Immunology, 23:760-769.
  • Jayaprakash C. and N. Shettu. 2013. Changes in the hematology of the freshwater fish, Channa punctatus (Bloch) exposed to the toxicity of deltamethrin. Journal of Chemical and Pharmaceutical Research, 5(6):178-183.
  • Kaya, H., Çelik, E.Ş., Yılmaz, S., Tulgar, A., Akbulut, M. and N. Demir. 2014. Hematological, serum biochemical and immunological responses in common carp (Cyprinus carpio) exposed to phosalone. Comparative Clinical Pathology, 24(3):497-507.
  • Mekkawy, I.A., Mahmoud, U.M., Saved A. and H. El-Din. 2011. Effects of 4-nonylphenol on blood cells of the African catfish Clarias gariepinus (Burchell, 1822), Tissue and Cell, 43:223-229.
  • Mgbenka, B.O., Oluah, N.S. and A.A. Arungwa. 2005. Erythropoietic response and hematological parameters in the catfish Clarias albopunctatus exposed to sublethal concentrations of actellic. Ecotoxicology and Environmental Safety, 62:436-440.
  • Mikula, P., Modra, H., Nemethova, D., Groch, L. and Z. Svobodova 2008. Effects of subchronic exposure to LASSO MTX® (Alachlor 42% W/V) on hematological indices and histology of the common carp, Cyprinus carpio L. Bulletin of Environmental Contamination and Toxicology, 81:475-479.
  • Modesto, K.A. and C.B.R. Martinez. 2010. Effects of roundup transorb on Fish: Hematology, antioxidant defenses and acetylcholinesterase activity. Chemosphere, 81:781-787.
  • Murad, A. and A.H. Houston. 1988. Leukocytes and leukopoietic capacity in goldfish, Carassius auratus, exposed to sublethal Levels of Cadmium. Aquatic Toxicology, 13:141-154.
  • Naveed, A., Venkaeshwarlu, P. and C. Janaiah. 2011. Biochemical alteration induced by triazophos in the blood plasma of fish, Channa punctatus (Bloch). Annals of Biological Research, 2(4):31-37.
  • Patnaik, L. and A.K. Patra. 2006. Haemoatopoietic alterations induced by carbaryl in Clarias batrachus (LINN). Journal of Applied Sciences and Environmental Management, 10(3):5-7.
  • Pavlidis, M., Futter, W.C., Katharios, P. and P. Divanach. 2007. Blood cell profile of six Mediterranean mariculture fish species. Journal of Applied Ichthyology, 23(1):70-73.
  • Ramesh, M. and M. Saravanan. 2008. Haematological and biochemical responses in a freshwater fish Cyprinus carpio exposed to chlorpyrifos. International Journal of Integrative Biology, 3(1):80-83.
  • Rehulka, J. and B. Minarik B. 2004. Effect of polychlorinated biphenyls (Delor 103) on haematological and enzyme parameters of the rainbow trout (Oncorhynchus mykiss). Diseases of Aquatic Organisms, 62:147-153.
  • Roy, B. and S. Nath. 2011. Some hematological investigations on Oreochromis niloticus (Trewavas) following exposure to thiamethoxam. Acta Zoologica Lituanica, 21(4):301-305.
  • Saravanan, M., Kumar, K.P. and M. Ramesh. 2011. Haematological and biochemical responses of freshwater teleost fish Cyprinus carpio (Actinopterygii: Cypriniformes) during acute and chronic sublethal exposure to lindane. Pesticide Biochemistry and Physiology, 100:206-211.
  • Satyanarayan, S., Bejankiwar, R.S., Chaudhari, P.R., Kotangale, J.P. and A. Satyanarayan. 2004. Impact of some chlorinated pesticides on the haematology of the fish Cyprinus carpio and Puntius ticto. Journal of Environmental Sciences, 16(4): 631-634.
  • Sepici Dinçel, A., Sarıkaya, R., Selvi, M., Şahin, D., Benli Ç.K. and S. Atalay Vural. 2007. How sublethal fenitrothion is toxic in carp (Cyprinus carpio L.) fingerlings. Toxicology Mechanisms and Methods, 17:489-495.
  • Shelly, L.K., Balfry, S.K., Ross, P.S. and C.J. Kennedy. 2009. Immunotoxicological effects of a sub-chronic exposure to selected current use pesticides in rainbow trout (Oncorhynchus mykiss). Aquatic Toxicology, 92:95-103.
  • Smith, C.J., Shaw, B.J. and R.D. Handy. 2007. Toxicity of single walled carbon nanotubes to rainbow trout (Oncorhynchus mykiss): Respiratory toxicity, organ pathologies, and other physiological effects. Aquatic Toxicology, 82:94–109.
  • Svobodova, Z. and M. Pecena. 1988. Changes in the red and White blood picture of carp after acute exposure to toxic substance. Bulletin Research Institute of Fish Culture and Hydrobiology (RIFCH) Vodnany, 17:116-128.
  • Thakur, G.K. and P.K. Pandey. 1990. BHC (Gammaxene [sic]) poisoning effect on leucocytes of an airbreathing fish, Clarias batrachus (Linn). Journal of Environmental Biology, 11:105-110.
  • Turner, L. 2002. Propargite analysis of risks to endangered and threatened salmon and steelhead. Retrieved July 08, 2013, from http://www.epa.gov/espp/litstatus/effects/propargite_analysis.pdf.
  • Val, A.L., De Menezes, G.C. and C.M. Wood. 1998. Red blood cell adrenergic responses in Amazonian teleost. Journal of Fish Biology, 52:83-93.
  • Velisek, J., Svobodova, Z. and J. Machova. 2009a. Effects of bifenthrin on some haematological, biochemical and histopathological parameters of common carp (Cyprinus carpio L.). Fish Physiology and Biochemistry, 35:583-590.
  • Velisek, J., Svobodova, Z., Piackova, V. and E. Sudova. 2009b. Effects of acute exposure to metribuzin on some hematological, biochemical and histopathological parameters of common carp (Cyprinus carpio L.). Bulletin of Environmental Contamination and Toxicology, 82:492-495.
  • Wedemeyer, G.A. 1997. Effects of rearing conditions on the health and physiological quality of fish in intensive culture. In: Iwama, G.K., Pickering, A.D., Sumpter, J.P. and C.B. Chreck. (eds). Fish stress and health in aquaculture. Cambridge University Press, Cambridge, p:35–71.
  • Witeska, M. 2005. Stress in fish – hematological and immunological effects of heavy metals. Electronic Journal of Ichthyology, 1:35–41.
  • Xu, S. 2001. Environmental fate of propargite. Retrieved July 08, 2013, from http://www.cdpr.ca.gov/docs/emon/pubs/fatememo/propargite.pdf.