2014 – Volume 3, Issue 2 / (Page 45-50)

Surface modification with P-aminohippuric acid on biogenic apatite (fish bones) particles

Evren Tan, Bayram Kizilkaya, Nail Ucyol, Hasan Basri Ormanci, Ayhan Oral



 The aim of this study with fish bones as a waste in fish processing is to investigate whether they can provide more efficient and multi-functional materials by chemical modification. As modification chemical, p-aminohippuric acid was selected and modified on the bone particle surface by esterification method. The results showed that surface modification was performed successfully. P-aminohippuric acid bonded on the surface of bone was calculated as 190.64 µmol/g. The point of zero charge (PZC) of bone apatite (H) and modified bone (HA5) was investigated and determined as 7.25 and 6.80, respectively. SEM-EDS spectrums showed that nitrogen element of p-aminohippuric acid on the surface of HA5 could be observed in EDS spectrum clearly. Additionally, nitrogen amount of Hand HA5 was detected as 8.037% and 8.565%, respectively.



 Fish bone, P-aminohippuric acid, Surface modification


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  • Alasbeb, S., Banat, F. and F. Mobai. 1999. Sorption of copper and nickel by spent animal bones. Chemosphere, 39(12):20872096.
  • Banat, F., Asheh, S.A. and F. Mohai. 2000. Batch zinc removal from aqueous solution using dried animal bones. Separation and Purification Technology, 21:155-164.
  • Corami, A., D’Acapito, F., Mignardi, S. and V. Ferini. 2008. Removal of Cu from aqueous solutions by synthetic hydroxyapatite: EXAFS investigation. Materials Science and Engineering B, 149:209–213.
  • Chojnacka, K. 2005. Equilibrium and kinetic modelling of chromium(III) sorption by animal bones. Chemosphere, 59:315–320.
  • Dimovic, S., Smiciklas, I., Plecas, I., Antonovic, D. and M. Mitric. 2009. Comparative study of differently treated animal bones for Co2+ removal. Journal of Hazardous Materials, 164:279–287.
  • El Hammari, L., Laghzizil, A., Saoiabi, A., Barboux, P. and M. Meyer. 2006. Chemical modification of porous calcium hydroxyapatite surfaces by grafting phenylphosphonic and phenylphosphite acids. Physicochemical and Engineering Aspects, 289:84–88.
  • Kizilkaya, B. and A.A. Tekinay. 2011. Comparative study and removal of Co and Ni (II) ions from aqueous solutions using fish bones. Science of Advanced Materials, 3:949–961.
  • Kizilkaya, B., Tekinay A.A. and Y. Dilgin. 2010. Adsorption and removal of Cu (II) ions from aqueous solution using pretreated fish bones. Desalination, 264:37–47.
  • Li, Y. and W. Weng. 2008. Surface modification of hydroxyapatite by stearic acid: characterization and in vitro behaviors. Journal of Materials Science: Materials in Medicine, 19:19– 25.
  • Murugan, R. and S. Ramakrishna. 2004. Coupling of therapeutic molecules onto surface modified coralline hydroxyapatite. Biomaterials, 25:3073–3080.
  • Ozawa, M., Satake, K. and R. Suzuki.2003. Removal of aqueous chromium by fish bone waste originated hydroxyapatite. Journal of Materials Science Letters, 22:513–514.
  • Smiciklas, I., Dimovic, S., Plecas, I. and M. Mitric. 2006. Removal of Co2+ from aqueous solutions by hydroxyapatite. Water Research, 40:2267–2274.
  • Jang, S.H., Jeong, Y.G., Min, B.G., Lyoo, W.S. and S.C. Lee. 2008. Preparation and lead ion removal property of hydroxyapatite/polyacrylamide composite hydrogels. Journal of Hazardous Materials, 159:294–299.
  • Zhu, R., Yu, R., Yao, J., Mao, D., Xing, C. and D. Wanga. 2008. Removal of Cd2+ from aqueous solutions by hydroxyapatite. Catalysis Today, 139: 94–99.
  • Hong, Z., Zhang, P., Liu, A., Chen, L., Chen, X. and X. Jing. 2006. Composites of poly(lactide-co-glycolide) and the surface modified carbonated hydroxyapatite nanoparticles. Journal of Biomedical Materials Research Part A, 81(3):515–522.
  • Wei, J., Liu, A., Chen, L., Zhang, P., Chen, X. and X. Jing. 2009. The surface modification of hydroxyapatite nanoparticles by the ring opening polymerization of g-benzyl-L-glutamate N-carboxyanhydride. Macromolecular Bioscience, 9:631– 638.